Molecular Evolutionary Genetics Analysis
Pattern among Lineages
This option becomes available if the distance model you have selected has formulas that
allow the relaxation of the assumption of homogeneity of substitution patterns among
lineages.
Rates among Sites
This option becomes available if the distance model you have selected has
formulas that allow rate variation among sites. If you choose gamma distributed
rates, then the Gamma parameter option becomes visible.
Bootstrap method to compute standard error of distance estimates
When you choose the bootstrap method for estimating the standard error, you must specify
the number of replicates and the seed for the psuedorandom number generator. In each
bootstrap replicate, the desired quantity is estimated and the standard deviation of the
original values are computed (see Nei and Kumar [2000], page 25 for details).
It is possible that in some bootstrap replicates the quantity you desire is not calculable for
statistical or technical reasons. In these cases, MEGA will discard the results of the
bootstrap replicates and its final estimate will be the results of all valid replicates. This
means that the number of bootstrap replicates used can be smaller than the number
specified by the user. However, if the number of valid bootstrap replicates is < 25, then
MEGA will report that the standard error cannot be computed (an "n/c" swill appear in the
result window).
5.23 Compute Pariwise
5.24 Compute Means
5.25 Compute Sequence Diversity
5.3 Constructing Phylogenetic Trees
5.31 Phylogenetic Inference
Reconstruction of the evolutionary history of genes and species is currently one of
the most important subjects in molecular evolution. If reliable phylogenies are
produced, they will shed light on the sequence of evolutionary events that
generated the present day diversity of genes and species and help us to understand
the mechanisms of evolution as well as the history of organisms.
Phylogenetic relationships of genes or organisms usually are presented in a treelike
form with a root, which is called a rooted tree. It also is possible to draw a tree
without a root, which is called an unrooted tree. The branching pattern of a tree is
called a topology.
Part IV: Evolutionary Analysis
There are numerous methods for constructing phylogenetic trees from molecular
data (Nei and Kumar 2000). They can be classified into Distance methods,
Parsimony methods, and Likelihood methods. These methods are explained in
Swofford et al. 1996, Li (1997), Page and Holmes (1998), and Nei and Kumar
(2000).
5.32 NJ/UPGMA Methods
Analysis Preferences (NJ/UPGMA)